Overcoming Intrinsic Restriction Enzyme Barriers Enhances Transformation Efficiency in Arthrospira platensis C1
نویسندگان
چکیده
منابع مشابه
Draft genome sequence of Arthrospira platensis C1 (PCC9438)
Arthrospira platensis is a cyanobacterium that is extensively cultivated outdoors on a large commercial scale for consumption as a food for humans and animals. It can be grown in monoculture under highly alkaline conditions, making it attractive for industrial production. Here we describe the complete genome sequence of A. platensis C1 strain and its annotation. The A. platensis C1 genome conta...
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The search for natural products with benefits for health in general and of potential for treating human disease has gained wider interest world-wide. Here, we analyse current data on the microalga Arthrospira platensis (AP), that has been used in nutrition since ancient times in Fare East and African communities, for medical purposes with a focus on dermatology. Extracts of AP have been investi...
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Arthrospira platensis is a multi-cellular and filamentous non-N2-fixing cyanobacterium that is capable of performing oxygenic photosynthesis. In this study, we determined the nearly complete genome sequence of A. platensis YZ. A. platensis YZ genome is a single, circular chromosome of 6.62 Mb in size. Phylogenetic and comparative genomic analyses revealed that A. platensis YZ was more closely r...
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در این تحقیق گونه خالص اسپیرولینا از اندونزی به منظور کشت به ایران منتقل شد. پس از بررسی های میکروسکوپی با استفاده از کلید شناسایی طبق شکل ظاهری، قطر و طول سلول، و اندازه تریکومهای جلبک سلول در محیط مایع و نیمه جامد به عنوان Arthrospira platensis شناسایی شد. جلبک در محیط آزمایشگاه طی 40 روز کشت داده شد و هر 2 روز یکبار تراکم سلولها محاسبه شد. ابتدا در ظروف 300ml و سپس در ظروف 5/1 لیتری درشرایط ...
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DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-GC5mCGGC-3' and 5'-GAG5mCTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the ...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 2017
ISSN: 0032-0781,1471-9053
DOI: 10.1093/pcp/pcx016